human PDGF-AA

  • 品牌:
  • 外观性状:白色粉状
  • 酶系活力:10^9
  • 温度适用范围:37
  • 产品标准:
  • 保质期:24

 
recombinant human pdgf-aa(short form)
specifications and use
sourcea dnasequence encoding the 110 amino acid residue mature human pdgf-achain peptide was expressed in yeastpichia pastoris. the recombinantpdgf-aa was glycosylated(pas staining).
molecularmass the disulfide-linkedhomodimeric rhpdgf-aa containing two 110 amino acid residuea chains with amolecular mass of approximately 34 kda.
purity> 98%as determined by sds-page analysis..
activitymeasuredby its ability to stimulate proliferation of mouse3t3fibroblasts. (pnas94,10205, 1997. biochemstry, 1996,35, 12077)
theed50for thiseffect is typically 1.0 ng/ml.
formulationlyophilized from a0.2 µm filtered solution in 20 mm sodium phosphate in absence ofany carrierproteins.
storagelyophilized samplesare stable for greater than 12 months at -80° c.
uponreconstitution, this growth factor can be stored under sterileconditions at 2° - 4° c for one month or at -20° c to -70° c forthree months without detectable loss of activity.
avoidrepeated freeze-thaw cycles.

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